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Development and validation of isocratic reversed-phase HPLC method for simultaneous estimation of torsemide and spironolactone in bulk and pharmaceutical combined tablet dosage form

S. SHARMA1, M. C. SHARMA2,* , S. C. CHATURVEDI3

Affiliation

  1. Department of Chemistry Yadhunath Mahavidyalya Bhind (M.P) 477001 India
  2. School of Pharmacy, Devi Ahilya Vishwavidyalaya, Indore (M.P)- 452001, India
  3. Shri Arvindo Institute of Pharmacy Ujjain Road Indore (M.P) 453111 India

Abstract

Validated high performance liquid chromatographic (HPLC) method for estimation of Torsemide (TOR) and Spironolactone (SPI) in tablet dosage form. Isocratic RP-HPLC separation was achieved on Licrosphere C18 column (250 x 4.6mm) using Methanol: Acetonitrile: Phosphate buffer, pH 6.5 (60:20:20 v/v) at flow rate of 1.5 ml/min at 30 oC temperature. Quantitation was achieved by UV detection at 252 nm over the conc. range 0-25 μg/ml for both the drugs with mean recoveries of 100.01% + 0.12 and 100.64% + 0.20 for TOR and SPI respectively. This method is simple, precise and sensitive and applicable for the simultaneous estimation of TOR and SPI in tablet pharmaceutical combined dosage form..

Keywords

Torsemide, Spironolactone, Pharmaceutical, HPLC method.

Citation

S. SHARMA, M. C. SHARMA, S. C. CHATURVEDI, Development and validation of isocratic reversed-phase HPLC method for simultaneous estimation of torsemide and spironolactone in bulk and pharmaceutical combined tablet dosage form, Optoelectronics and Advanced Materials - Rapid Communications, 4, 3, March 2010, pp.427-430 (2010).

Submitted at: Feb. 10, 2010

Accepted at: March 12, 2010