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Evaluation of antibacterial effect on Escherichia coli PTCC 1395 of TiO2 nanoparticles synthesized via sol-gel method

ALI ABDOLAHZADEH ZIABARI1,* , SAJIHE BAHREKAZEMI2

Affiliation

  1. Department of Physics, Faculty of Science, Lahijan Branch, Islamic Azad University, P.O. Box1616 , Lahijan, Iran
  2. Department of Microbiology, Faculty of Science, Lahijan Branch, Islamic Azad University, Lahijan, Iran

Abstract

Titanium dioxide (TiO2) nanoparticles were synthesized by sol-gel method and their physico-chemical properties were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Using the well-known Scherrer formula, the size of nanoparticles was estimated. This was 28.70, 31.05 and 32.94 nm for 0.1, 0.3 and 1 molar nanoparticle concentrations, respectively. The TiO2 nanoparticles prepared at 0.1 M had only anatase phase, but at 0.3 and 1 M both anatase and rutile phases were observed. The photocatalytic property of TiO2 nanoparticles was investigated by inactivation of Escherichia coli PTCC 1395 under irradiation by a UV-B lamp. The sharp peaks in FTIR spectrum indicated the purity of TiO2 nanoparticles. The TiO2 nanoparticles have a significant antibacterial effect on Escherichia coli PTCC 1395 at concentration of 1mg/mL. Increasing the molarity from 0.1 to 1 M, decreased the viable cell concentration of Escherichia coli PTCC 1395 from 88.89 % to 8.33 % after 3 hours..

Keywords

Escherichia coli PTCC 1395, Titanium dioxide, nanoparticle, Sol-gel, Photocatalytic.

Citation

ALI ABDOLAHZADEH ZIABARI, SAJIHE BAHREKAZEMI, Evaluation of antibacterial effect on Escherichia coli PTCC 1395 of TiO2 nanoparticles synthesized via sol-gel method, Optoelectronics and Advanced Materials - Rapid Communications, 8, 3-4, March-April 2014, pp.230-234 (2014).

Submitted at: Nov. 2, 2013

Accepted at: March 13, 2014